Goals of the BRAIN 3D Microscopy Standards Project

       Our goal is to provide the neuroscience research community with a set of standards for 3D microscopy of intact brains. The proposed standards can help facilitate:
  •        Consistency of data collection and reporting
  •        Evaluation, validation, and interpretation of microscopy experiments
  •        Development of data repositories and computational tools
  •        Leveraging multiple datasets to gain novel insights into brain biology


Proposal Specific Aims

  • Specific Aim 1: Establish project operating parameters and assemble a WG of 3D brain microscopy experts.
  • Specific Aim 2: Develop preliminary standards using a consensus process that relies on input from the research community.
  • Specific Aim 3: Raise awareness and promote uptake of 3D brain microscopy standards.


BRAIN 3D Microscopy Working Group Scope

Sample Preparation

Tissues Clearing

Image Acquisition

Processing and Analysis

  • Organism 
  • Labeling 
  • Tissue collection
  • Technique
  • Transparency
  • Technique
  • Equipment
  • Wavelengths
  • Resolution
  • File format
  • Software
  • 3D reconstruction